13, 176 (2021). He also has a special interest in management of wisdom teeth and dental implants with general anesthesia. 2). Sci. Click on the address below to send us an email. We genuinely care about your well-being and want to make sure your oral health improves following your procedure. A. et al. 8, 760772 (2015). Bioinform. 4b), while N. flavescens (p<0.003; Pr > 0.4) was the most prevalent and negatively associated with the oral samples (Supplementary Table 4). Providers Overview Location Reviews Providers Dr. Jung Ahn, DMD Dentistry 0 Ratings Dr. Brandie Ard, DMD Dentistry 1 Rating Dr. Sarah Iqbal, DMD Dentistry 4 Ratings Dr. Freddie Wakefield, DDS Sedation Dentistry Together, these genes may constitute relevant markers of the presence of bacteria that respond to the stressful conditions generated by HIV disease. Here we performed a metagenomics-based characterization of the oral and anal microbiome in a cohort of MSM and TGW patients exposed to HIV infection to investigate their relationship with associated factors such as gender, sexual behavior, ART, viral load, CD4+ T-cell counts and the presence of precancerous anal intraepithelial lesions. At Associated Oral & Maxillofacial Surgeons, we know how to help you. Katie Hobbs said the rule will go into effect immediately. Founded in 1846, AP today remains the most trusted source of fast, accurate, unbiased news in all formats and the essential provider of the technology and services vital to the news business. Furthermore, the depletion of energetic bioprocesses would be related to the nutritional deficits observed in HIV disease82. C. comes and H. biformis were among the depleted bacteria in HIV-positive compared with HIV-negative (Fig. Furthermore, our results confirm previous results from other studies and provide new insights that need to be explored in future studies. Alterations in the gut microbiota associated with HIV-1 infection. Clin. Furthermore, we estimated the relative risk (RR) associated with the presence of lesions in subjects with P. bivia and F. gonidiaformans in anal samples compared with individuals without detection of these taxa. Guilln, Y. et al. The oral virome of our cohort showed enrichment in bacteriophages, KSHV (HHV-8) and HHV-7, among others, being KSHV significantly predominant in HIV-positive and TGW independently of sexual behavior, compared with HIV-negative and MSM, respectively. Lancet Infect. By default, gene families are annotated using the comprehensive protein database UniRef9092 and metabolic pathways are annotated using MetaCyc database93. Mucosal Immunol. 279, 5234652352 (2004). Boletn sobre el VIH, sida e ITS en la Argentina. Mucosal Immunol. To further identify bacteria species related to HIV variables, we applied MaAsLin2 considering gender, CSW status, presence of lesions, current CD4+ T-cell counts, and current VL. and JavaScript. The Associated Press is an independent global news organization dedicated to factual reporting. 2). We considered the observed species and Chao1 indices for richness, and the Shannon and Simpson indices for diversity. 2000 87, 107131 (2021). Baseline information on oral health, associated with adequate preventive procedures, is fundamental to promote self-preventive behavior. the oral cancers in India progress from leukoplakia [4]. Heatmap representation of taxa abundances was done with the R package phyloseq. Conversely, anal bacteria showed a significant decrease in HIV . We used default parameters for normalization (TSS method), transformation (Log), analysis method (LM), correction method (BH), and significance threshold (q-value<0.25). Associated Oral and Implant Surgeons offers state-of-the-art surgical facilities with the most current sterilization, anesthesia monitoring, and imaging equipment at their three locations in Johnson City, Kingsport, and Bristol, TN. The images or other third party material in this article are included in the articles Creative Commons license, unless indicated otherwise in a credit line to the material. Although he could not review the final version of this manuscript before submission, it is important to acknowledge his crucial role in reviewing all preliminary versions. Remarkably, when we considered those co-infected with the two species the RR increased to 9.5 (RR=9.5; 95%CI=1.447862.7231; p=0.019) compared with those without none of both bacteria (Table 3). Article J. Infect. Three viral species were found positively associated with the low-CD4+ T-cell counts group: Haemophilus phage Aaphi23, Streptococcus phage MM1, and the Human alphaherpesvirus 2 (HSV-2) (Fig. & Olasehinde, G. I. : Conceived the study, funding acquisition, supervision, methodology, manuscript review, and editing. We want to express our heartfelt appreciation for the invaluable contributions of Dr. Enrique Mesri, the core and soul of the UM/SCCCArgentina Consortium for research and training in Virally Induced AIDS Malignancies. The staff is committed to providing high-quality, compassionate care in a timely and considerate manner. WASHINGTON (AP) U.S. officials have approved the first over-the-counter birth control pill, which will let American women and girls buy contraceptive medication from the same aisle as aspirin and eyedrops. ASSOCIATED ORAL AND MAXILLOFACIAL SURGEONS - 12 Photos - 501 Main St, Elk River, Minnesota - Oral Surgeons - Phone Number - Yelp Associated Oral and Maxillofacial Surgeons 3 reviews Unclaimed Oral Surgeons Closed 7:00 AM - 3:30 PM See hours Write a review Add photo Photos & videos See all 12 photos Add photo You Might Also Consider Sponsored However, NMR-r in patients . PLoS ONE 8, e61217 (2013). Hussan, H., Clinton, S. K., Roberts, K. & Bailey, M. T. Fusobacteriums link to colorectal neoplasia sequenced: a systematic review and future insights. Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page. J. Virol. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. (423) 915-1072 Long, X. et al. We were able to define gene signatures related to HIV infection, the presence of lesions and detectable viral load (Fig. McClelland, R. S. et al. ASSOCIATED ORAL & MAXILLOFACIAL SURGEONS - 6550 Mercantile Dr E, Frederick, Maryland - Oral Surgeons - Phone Number - Yelp Associated Oral & Maxillofacial Surgeons Claimed Oral Surgeons Closed See hours Write a review Add photo Photos & videos See all 5 photos Add photo Services Offered Verified by Business Virtual Consultations Showing 1-1 of 1 Location . b VL detectable vs. VL undetectable; reference value=VL detectable. However, little is known about the relationship between oral bacteria and respiratory outcomes in the general population. A recent study revealed that mucosal bacteria could predict the presence of anal lesions in HIV-infected MSM79. Correspondence to Arteries that move blood from the heart to the face and oral cavity meet up with nerves from the brain that innervate the face and oral cavity. Gootenberg, D. B., Paer, J. M., Luevano, J. M. & Kwon, D. S. HIV-associated changes in the enteric microbial community: potential role in loss of homeostasis and development of systemic inflammation. Microbiome 4, 58 (2016). These pathways involve, the biosynthesis of amino acids, pyruvate metabolism, glycolysis and lipid biosynthesis among others. Beta diversity was measured by BrayCurtis, weighted UniFrac, and unweighted UniFrac. Infect. Tobacco use is the main risk factor for HNSCC, and tobacco-associated HNSCCs have poor prognosis. Sexual behavior variables comprised Age at First Sexual Intercourse (AFSI), Age of Anal Sex Initiation (AASI), Age of Oral Sex Initiation (AOFSI), Number of Sexual Partners in the Last Month (NSPLM), Number of Sexual Partners in Life (NSPL), Condom Use in Anal (CUAS) and Oral Sex (CUOS), and whether participants are or have been Commercial Sex Workers (CSW). 6b), suggesting these genes could be relevant markers of ART treatment response. Introduction Oral health is a crucial yet often neglected aspect of rehabilitation. 37. The diagnosis of OSMF was based on difficulty in opening the mouth and associated blanched oral mucosa, with palpable fibrous bands. With a dual-certified dentist and medical physician in-house, Associated Oral & Maxillofacial Surgeons offers patients unique insight into conditions that other dentists may overlook. Background: The oral cavity is the gateway to the bacteria community in the lung. Consumption habits included Alcohol, Tobacco, and intravenous or non-intravenous drug use. a Heatmap representation of the fifty-two PWYs associated with oral and anal sites contributed by fifty-four species. Importantly, HIV-positive cases also showed more abundance of Alphapapillomavirus 9 which contains the high-risk genital cancer HPV16, independently of gender condition (Fig. 6c). To identify features that differ between sample categories, variables were defined and dichotomized as follows: site (oral/anal); gender (TGW/MSM); age (based on the median, under33yo/over33yo); CSW (yes/no); HIV status (positive/negative); current CD4+ T-cell counts (<500cel/mm3/>500cel/mm3); current VL (detectable: >50copies/ml, undetectable:<50copies/ml); for anal cytology, we grouped ASCUS (n=3), LSILs (n=40) and HSILs (n=8) into the variable name presence of lesions (yes/no). Of note, all were increased in TGW compared with MSM. This suggests that this population may be at greater risk of acquiring sexually transmitted infections. Gut Microbes 13, 1997292 (2021). Pathways multivariable analysis revealed a significant enrichment of several metabolic processes in younger subjects, TGW, CSW, and subjects with precancerous anal lesions, all groups characterized by subjects with high frequency of sexual intercourse. J. Curr. Dis. DR. RYAN DOWLING, JOHNSON CITY OFFICE a Box plots showing the significant relationship of four anal bacterial species with CD4+ T-cell counts. 5b). PubMed Central Although association in MaAsLin 2.0 is considered significant at a q-value of below 0.25, a cut-off used in previous microbiome studies, for more stringency we selected the associated features (species, pathways, gene families) with a q-value<0.15 (nominal p-value<0.05)86,89,90,91. Res. In addition to digital X-rays and cone-beam computed tomography (CBCT) scans, Associated Oral & Maxillofacial Surgeons offers patients titanium and zirconia implants from top providers, like ZimVie. Vzquez-Castellanos, J. F. et al. Oxidative stress during HIV Infection: mechanisms and consequences. Lymphotropic viruses EBV, KSHV and HTLV in Latin America: epidemiology and associated malignancies. Anaerobe 32, 3742 (2015). 107 Newtown Rd., Suite 1A, Danbury, CT 06810, 901 Ethan Allen Hwy, Suite 203, Ridgefield, CT 06877, 7 Garage Rd, Suite B, Southbury, CT 06488, Address: 107 Newtown Rd., Suite 1A, Danbury, CT 06810, Along with traditional single implants, the team offers, The practice proudly offers the latest technologies and materials to perform dental surgery. 11, 2448 (2020). American Association Of Oral & Maxillofacial Surgeons is a Oral Surgeon facility at 812 Central Road in Bloomsburg, PA. Services: American Association Of Oral & Maxillofacial Surgeons is an Oral Surgeon located in Bloomsburg, PA. An Oral Surgeon is trained to diagnose, treat, and prevent diseases of the gums, teeth, and jaw. There are several actions that could trigger this block including submitting a certain word or phrase, a SQL command or malformed data. Biol. ASSOCIATED PRESS. Details. A new study followed a patient with primary sclerosing cholangitis (PSC) and associated ulcerative colitis (UC) who was started on 500 mg of oral vancomycin twice daily because he could not achieve remission of his UC on advanced therapies. Pathways analysis revealed a significant decrease of several pathways in HIV-positive patients, mainly contributed by anal bacteria. Intestinal microbial communities and Holdemanella isolated from HIV+/- men who have sex with men increase frequencies of lamina propria CCR5+ CD4+ T cells. For educating and motivating general public, it is of great importance that the . Curty, G. et al. Prognostic Role of CD68+ and CD163+ Tumour-Associated - PubMed & Wu, C. H. UniProt Consortium. 12, 232246 (2019). Characteristics and dysbiosis of the gut microbiome in renal transplant recipients. We can also diagnose and treat facial pain and facial injuries, and perform a full range of dental implant and bone grafting procedures. : methodology, manuscript review. Dentist accused of poisoning wife searched - Associated Press News AIDS 32, 23632371 (2018). PLoS Pathog. M.E.S. PLoS ONE 9, e106078 (2014). Overall, distinctive microbial compositions were identified in oral and anal mucosa of a cohort of MSM and TGW HIV-positive and negative cases, with a greater richness at the species level in the oral samples. M. indolicus is a recently described species originally isolated from the female genital tract74. 104 were here. It is established that HIV infection is associated with a greater HPV capacity to persist and increase the risk and progression of precancerous anal intraepithelial lesions. The HIV epidemic in Latin America: a time to reflect on the history of success and the challenges ahead. In particular, TGW suffer social exclusion at an early age, limiting their access to education and jobs, leading many TGW to engage in sex work16,17. Differential effects of antiretrovirals on microbial translocation and gut microbiota composition of HIV-infected patients. Protein Sci. 3, 162 (2020). In contrast, Bacteroidetes (p<0.0001), Spirochaetes (p<0.01), the unusual group Tenericutes (p<0.01) and Viruses (p<0.001) were most abundant in anal samples (Supplementary Fig. Introduction: Dental health and oral health are used almost synonymously when stating the goals of oral health; such statements are only valid for dental health. Suzek, B. E., Wang, Y., Huang, H., McGarvey, P. B. Austin, M. N. et al. J. Immunol. Mutlu, E. A. et al. All participants included in this study signed informed consent before being involved in the project. Dental Implants | Associated Oral & Maxillofacial Surgeons Associated Oral & Maxillofacial Surgeons Office Locations . For viruses, species of Alphapapillomavirus were prevalent in anal samples, while Human gammaherpesvirus 8 (KSHV) and Human betaherpesvirus 7 (HHV-7) along with bacteriophages of Actynomyces and Streptococcus, among others, were dominant in oral samples (Fig. S.W. McMurdie, P. J. These species differed from those found associated with HIV infection, and were all enriched in TGW compared with MSM, and many were also prevalent in the group of CSW. His primary clinical interests include wisdom teeth removal, dentoalveolar surgery, dental implants, oral pathology and maxillofacial trauma. O.C. Shin, D. H. et al. 929 W Carl Sandburg Dr . ASSOCIATED ORAL & MAXILLOFACIAL SURGEONS - 6550 Mercantile Dr E - Yelp Miss, D. et al. Bai, X. et al. "Beyond the Story: 10-Year Record of BTS" is a 544-page, glossy oral history of the world's biggest boy band by Myeongseok Kang and BTS for Flatiron Books. Together, our results and those found in other studies indicate that C. comes and H. biformis could potentially accelerate HIV progression while the patient is not receiving treatment, placing them as potential markers of the immune response to ART treatment in HIV-infected patients. Cite this article. Dr. Ryan Dowling MD, DMD, graduated from University of Connecticut School of Dentistry and earned his Doctor of Dental Medicine. In anal samples, species of Prevotella (P. copri, P. corporis, P. bivia, P. disiens, etc.) : funding acquisition, supervision, manuscript review M.C.A. Heatmap visualization of differentially represented taxa and pathways was done with R/Bioconductor and the MultiExperiment Viewer software (MeV v4.9). The most potent carcinogens in tobacco are specific nitrosamine and Dr. Larry Otte, DMD. As the majority of TGW included in this study are or have been CSW (30/31) while MSM have this condition in a significantly lower proportion (6/38), we hypothesized that TGW-related taxa could be linked to sexual behavior. 19, 390 (2019). PubMed The UniRef90 gene family abundance from HUMAnN3 was then regrouped to Kyoto Encyclopedia of Genes and Genomes (KEGG) orthology (KO)94. Dis. Gene family analysis identified bacterial gene signatures associated with HIV status, viral load, ART, and the presence of lesions, which may have potential as prognostic and predictive biomarkers of HIV/AIDS-associated malignancies. Moreover, it has recently observed to be significantly higher in samples with cervical intraepithelial neoplasia (CIN), creating a plausible environment for subsequent HPV infection, recurrence of CIN and cancer development37. J. Periodontol. It is important to note that none of the TGW included in the study have undergone gender-affirming surgery. Yellow arrowheads point to the most significant taxa shared by TGW and CSW. Lu, W. et al. A decrease in alpha diversity is observed in the group of older subjects, HIV-positive patients and subjects with lesions (p<0.05). Click to reveal Health- and disease-associated species clusters in complex natural biofilms determine the innate immune response in oral epithelial cells during biofilm maturation. Of these genes, the so-called moonlighting genes (which have more than one biological function), DNAk and ENO, stand out. Commun. Oral Surgery Sunbury and Lewisburg PA, Oral Surgery Dis. For HIV-positive, we identified gene signatures related to oxidative and energy metabolism. Most of them (26/39) showed a significant decrease in their relative abundances in HIV-positive compared with HIV-negative (p<0.05) and belong mostly to the anal site. The latter is a common pathogen co-infecting more than half of HIV-positive adults and has been postulated to accelerate HIV disease24. Google Scholar. These studies suggest C. comes plays a relevant role in the initial phase of HIV infection, modulating acute phase response with pro- or anti-inflammatory factors to manipulate the immune system. 2d). Liu, Y. et al. PDF The Occurrence of Potentially Malignant Disorders in the Oral - IJBM The team can diagnose and treat facial injuries, facial pain, and temporomandibular joint (TMJ) disorders, and perform a full range of implant and bone grafting procedures. Dentists remain to have a crucial role in the early diagnosis and treatment of HIV-associated oral lesions during COVID-19 pandemic that will have an impact on HIV treatments, also in implementing the Bali Declaration on oral health in HIV/AIDS 2019 to support UNAIDS goal to end AIDS by 2030. Conversely, a decrease of Firmicutes (p=0.0069) and Actinobacteria (p=0.0001) was observed in anal samples (Supplementary Fig. 1b). 2807 N Knoxville Ave Peoria, IL 61604. Cell Infect. You can email the site owner to let them know you were blocked. Thank you for visiting nature.com. While the microbiome has been well documented in the gut mucosa, less is known about the oral mucosa in the HIV context, particularly in TGW31,70. In this sense, our results reveal two related but different settings: on one side the enrichment of several metabolic PWYs associated with younger subjects, TGW, Commercial Sex Workers, and subjects with precancerous anal lesions; on the other a predominant decrease in various pathways associated with people with HIV, detectable VL, ART treatment, and low CD4 cell counts, variables more related to the HIV condition (Fig. Prior to dental school he concurrently earned a Bachelors of Science Degree in Physician Assistant studies and a Masters of Public Health Degree from Nova Southeastern University. For HIV group, only Viruses yielded a positive association with anal samples of the infected patients (p=0.038, Additional file 5). Medicare Accepted: Yes. In addition, HPV18 and HPV72 were more prevalent in TGW compared with MSM. Interestingly, we also identified a signature of oral species associated with gender and/or sexual behavior independently of HIV condition. In the bottom, it is indicated which genes are also differentially abundant among ART treated vs. ART untreated. Shotgun metagenomics sequencing allowed us to define viral and bacterial taxa associated with the subjects gender, sex behavior, HIV infection, and related variables such as CD4+ T-cell counts, HIV viral load, and the development of precancerous anal lesions. volume9, Articlenumber:48 (2023) PubMed Although many of these taxa have been previously characterized in the context of periodontal processes, little is known about their association with HIV. Dr. Hamlin is a full scope oral and maxillofacial surgeon with expertise ranging from dental implants to corrective jaw surgery, facial injuries, wisdom teeth removal, and pediatric and adolescent oral surgery. Microbiol 11, 769950 (2022). Thus, since these HPV types would be related to SILs, HPV16 would also be favored by HIV-positive conditions, constituting a potential determinant of anal lesions in HIV-infected patients23. For coefficients, referred to as the contrast between the category specified versus the reference category, we considered as reference category the first term or numerator of the dichotomous annotation89. PLoS Pathog. Kanehisa, M. & Goto, S. KEGG: kyoto encyclopedia of genes and genomes. 37 were here. Peoria Office | Associated Oral & Maxillofacial Surgeons Peoria Office 2807 N Knoxville Ave. Peoria, IL 61604 Directions (309) 326-9207 Monday: 8:00a.m. Prevotella bivia and Fusobacterium gonidiaformans were positively associated with anal precancerous lesions among HIV-infected subjects. Research of the potential biomarkers in vaginal microbiome for persistent high-risk human papillomavirus infection. In addition, H. biformis was enriched in untreated HIV-positive MSM or on a diversity of different ART drugs43. More than half the world's population sees AP journalism . Associated Oral Surgeons is a medical group practice located in Peoria, IL that specializes in Anesthesiology and Nurse Anesthesiology. 1144 Opal Ct . Oral anticoagulant prescription rates poor after newly - Healio Your oral surgeon will duplicate the model's position, depth, and other features in the surgery. He, L. & He, J. Ann. PubMedGoogle Scholar. Furthermore, among the most enriched bacteria in the oral mucosa of TGW was F. nucleatum, a periodontal pathogen that can promote cancer by several mechanisms. Source: Uppsala University. de Salud M., de la Nacin P., 2020. C. ureolyticus has been identified as an emerging gastrointestinal pathogen causative of inflammation, gastroenteritis and diarrhea in patients with HIV infection40,41. Dillon, S. M., Frank, D. N. & Wilson, C. C. The gut microbiome and HIV-1 pathogenesis: a two-way street. 1d). Socas, M. E. et al. Hence there was a need for a study to know the diversity in tobacco usage and their associated oral mucosal lesions. DNA samples were subject to cleanup and then processed for shotgun metagenomics sequencing using the PCR-free KAPA HyperPLUS (Roche, France) library preparation kit at the Onco-Genomics Shared Resource (OGSR, University of Miami, USA). In both heatmaps taxa are ordered according to prevalence and abundance; samples were clustered by an unsupervised method. Nucleic Acids Res. Infect. It has been related to oral squamous cell carcinoma and colorectal cancer78. For AIDS-associated malignancies such as KS, the effect of antiretroviral therapy (ART) has been dramatic, with significant decreases in incidence and outcomes improvement. Virome of anal samples was mainly represented by species of Alphapapillomavirus, being Alphapapillomavirus 10 (HPV6, HPV11), 9 (HPV16), 3 (HPV72), and 7 (HPV18) among the most prevalent. Further information on research design is available in the Nature Research Reporting Summary linked to this article. In previous studies, taxa of these families have been found mostly depleted in HIV infection62,63,64,65,66. 16, e1008524 (2020). 197, 13941401 (2008). The Food and Drug Administration said Thursday it cleared Perrigo's once-a-day Opill to be sold without a prescription, making it the . Ding, T. & Schloss, P. D. Dynamics and associations of microbial community types across the human body. Herpes simplex virus type 2 coinfection does not accelerate CD4 count decline in untreated HIV infection. All authors contributed to the article and approved the submitted version. Other diagnostic features included burning sensation, salivation, tongue protrusion, habits, and associated malignant changes. Importantly, no study to date has comprehensively delineated the oral and anal microbiome portrait of TGW with and without HIV, which is the primary aim of the present study20. P.C. PubMed Peptostreptococcus anaerobius promotes colorectal carcinogenesis and modulates tumour immunity. 6d). In Argentina, the burden of HIV infection among MSM is 14%, while in TGW has been reported as high as 34% compared to 0.4% in the general population15. Yamada, E. et al. On the other hand, genes identified in the group of subjects with lesions were all enriched in this group (Fig. Associated Oral Specialties | Atlanta GA - Facebook Ivanov, A. V. et al. Similarly, C. comes was found to be enriched in a group of HIV-infected patients categorized as immunological ART non-responders compared to the group of patients immunologically responsive to treatment44.